Application

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected ARID1B in HeLa, A431, HUVEC and NIH/3T3 cells.

Anti-ARID1B, clone 3D9.1, Cat. No. MABN2266, is a highly specific mouse monoclonal antibody that targets AT-rich interactive domain-containing protein 1B and has been tested for use in Immunocytochemistry and Western Blotting.

General description

AT-rich interactive domain-containing protein 1B (UniProt: Q8NFD5; also known as ARID domain-containing protein 1B, BRG1-associated factor 250b, BAF250B, BRG1-binding protein hELD/OSA1, Osa homolog 2, hOsa2, p250R) is encoded by the ARID1B (also known as BAF250B, DAN15, KIAA1235, OSA2) gene (Gene ID: 57492) in human. ARID domain-containing protein 1B (ARID1B) is widely expressed with high levels in heart, skeletal muscle, and kidney. Four isoform of ARID1B have been reported that are produced by alternative splicing. Its ARID domain is localized to amino acids 1053-1144. ARID1B is a component of the SWI/SNF chromatin remodeling complex and may play a role in cell-cycle activation. It is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). ARID1B and ARID1A display about 60% homology, but have been reported to have opposing functions in cell cycle arrest and are mutually exclusive since individual SWI/SNF chromatin remodeling complexes can contain either ARID1A or ARID1B, but not both. ARID1B belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. Mutations in ARID1B gene are linked to the development of Coffin-Siris syndrome 1, a congenital multiple malformation syndrome with intellectual disability, coarse facial features, and malformations of the cardiac, gastrointestinal, genitourinary, and/or central nervous systems.

Immunogen

GST/His-tagged recombinant fragment corresponding to 138 amino acids from the N-terminal half of human AT-rich interactive domain-containing protein 1B.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ARID1B in 10 µg of HeLa cell lysate.

Specificity

Clone 3D9.1 detects AT-rich interactive domain-containing protein 1B (ARID1B) in human and murine cells. It targets an epitope within 138 amino acids from the N-terminal half.

Target description

~300 kDa observed; 236.12 kDa calculated. Uncharacterized bands may be observed in some lysate(s).